Artigos e Publicações

Latin America: Situation and preparedness facing the multi-country human monkeypox outbreak

By June 28, 2022, at least 48 cases in seven Latin American countries (Argentina, Brazil, Chile, Colombia, Mexico, Peru, and Venezuela) have been PCR-confirmed, with at least 16 additional suspected cases. Most countries in the region have settled their epidemiological surveillance to detect probable and suspected cases according to national and international case definitions (by the

Monkeypox: Some Keys to Understand This Emerging Disease

Monkeypox has entered our lives when we have not yet fully recovered from the SARS-CoV-2 pandemic. This close relative of the smallpox virus was restricted to tropical Africa, except on rare occasions when it caused human cases in the US, UK, Singapore, or Israel. Cases have recently been identified in multiple countries in at least

A bioluminescent and homogeneous SARS-CoV-2 spike RBD and hACE2 interaction assay for antiviral screening and monitoring patient neutralizing antibody levels

Here we describe a homogeneous bioluminescent immunoassay based on the interaction between Fc-tagged SARS-CoV-2 Spike RBD and human ACE2, and its detection by secondary antibodies labeled with NanoLuc luciferase fragments LgBit and SmBit. The assay utility for the discovery of novel inhibitors was demonstrated with a panel of anti-RBD antibodies, ACE2-derived miniproteins and soluble ACE2.

COVID-19 diagnosis by RT-qPCR in alternative specimens

Abstract Background: The high demand for adequate material for the gold standard reverse transcription real-time polymerase chain reaction (RT-qPCR)-based diagnosis imposed by the Coronavirus disease 2019 (COVID-19) pandemic, combined with the inherent contamination risks for healthcare workers during nasopharyngeal swab (NP) sample collection and the discomfort it causes patients, brought the need to identify alternative specimens